The intracellular polysaccharides of Agaricus blazei Murill by submerged culture were extracted by enzyme hydrolysis method. 以液体深层发酵培养的姬松茸菌丝体为原料,利用酶法提取姬松茸胞内多糖。
Quantitative analysis of intracellular cholesterol content was performed by cholesterol enzyme link assays. The expression of LDLR mRNA was detected by reverse transcription polymerase chain reaction ( RT-PCR) and LDLR protein was detected by Western blot. 通过胆固醇酶联法测定细胞内胆固醇含量,逆转录&聚合酶链反应检测细胞低密度脂蛋白受体mRNA的表达,westernblot检测细胞低密度脂蛋白受体蛋白的表达。
The trehalose synthase of S1 was detected to be an intracellular enzyme with the activity of 7.30 u/ g wet cells. 并且通过实验发现菌株S1的海藻糖合成酶为胞内酶,酶活力为7.30u/g菌体。
Through the studies on biochemical mechanism, the malolactic conversion mechanism was proposed that MLF was catalysed by LAB intracellular malolactic enzyme ( MLE). 通过对MLF生化机理的研究,提出了MLF是LAB利用胞内苹果酸-乳酸酶(MLE)直接催化苹果酸转变成乳酸的过程。
Effects of Osmotic Stress on Intracellular Glycerol Content and Enzyme Activity in Dunaliella salina 渗透胁迫对杜氏盐藻胞内甘油含量及相关酶活性影响
The fixed macrophages were stained by sudan ⅳ and quantitative analysis of intracellular cholesterol content was performed by cholesterol enzyme assays. 固定后的细胞以苏丹Ⅳ染色,观察细胞内脂质含量变化。通过胆固醇酶联法测定细胞内胆固醇含量。
Various neurotransmitters activate the membrane receptor proteins or intracellular enzyme pathways and result in the production of extracellular chemical signals. Second messenger molecules and ions transmit and amplify the signals, and subsequently induce the relaxation of smooth muscle cells and penile erection. 各种神经递质通过活化细胞膜受体蛋白或细胞内酶途径产生细胞外化学信号,细胞内第二信使分子和离子传递并放大这些信号,使平滑肌细胞舒张,最终诱发勃起。
The release of intracellular album clastic enzyme resulted in yeast autolysis and the causations of yeast autolysis covered: 1.yeast species; 酵母自溶由酵母胞内蛋白分解酶外泄引起,影响酵母自溶的因素有:(1)酵母菌种;
Effects of vitamin E supplementation on intracellular antioxidant enzyme production in adolescents with type 1 diabetes and early microangiopathy 1型糖尿病和早期微血管病的青少年患者补充维生素E对细胞内抗氧化酶合成的影响
In order to study the relationship of muscle injury, the release of intracellular enzyme, muscle mitochondrial [ Ca 2+] ( MCC) and lipid peroxidation through the stretch of rat soleus in vitro. 通过大鼠比目鱼肌体外实验,探讨牵拉负荷导致的肌肉损伤与肌细胞内酶的流出,骨骼肌细胞线粒体钙浓度(MCC)以及细胞脂质过氧化反应之间的关系。
Microalgae kept a proper survival rate in different environment stress by changing cell size, accumulating intracellular ROS, regulating enzyme synthesis and photosynthesis. 微藻在各种环境压力下,通过改变细胞大小,积累胞内ROS,调控相关酶合成和调节光合作用过程,从而保证在恶劣条件下的一定生存率。
Curdlan was synthesized under nitrogen-limited condition, the synthesis and activity of intracellular enzyme was greatly restrained at the condition, leading to the decreasing productivity of curdlan and the hard control of fermentation process. 由于氮源的缺乏,使得热凝胶合成期间细胞内相关酶的合成及其活性的恢复受到极大的限制,造成热凝胶合成速率的降低和发酵过程控制的困难。
However, the major problem was that the total enzyme activity was not very high and the extracellular CAT production was low, requiring great cost for cell rupture in order to obtain the intracellular enzyme. 其主要问题是总酶活相对较低,且胞外CAT产量不高,经细胞破碎获取胞内酶成本较高,不适合工业化生产的应用。
It has made substantial contribution to the understanding of many phenomena, such as intracellular enzyme activities, intracellular flux, and cell-cell interactions, since it originated over one century ago. 一个世纪以来,细胞培养在人们解释许多生物现象上做出了很大的贡献,比如包内酶活性、细胞内代谢通量、细胞与细胞之间的相互作用等等。
Many microfluidic devices have been developed for cell culture and to study intracellular enzyme activities, cellular responses to chemical gradients, cellular differentiation, dynamic gene expression and cell cytotoxicity screening. 许多微流控细胞阵列已经被用于研究包内酶活性、化学浓度梯度对细胞的刺激、细胞分化、基因表达的动力学特征和高通量药物筛选,且都在纳升或皮升级下进行。
Research was carried on POD and SOD activity and their intracellular distribution characteristics in sugar beet and BNYVV interaction processes by using biochemical detection and enzyme cytochemical methods. 采用生化检测及酶细胞化学方法研究了甜菜与BNYVV互作过程中POD和SOD的活性及其在细胞内分布特征。
This is particularly important for Mycobacterium tuberculosis and other intracellular pathogens, fpg is the key enzyme in base excision repair. 碱基切除修复对结核分枝杆菌等胞内致病菌尤其重要。fpg是碱基切除修复的关键酶。